Median arcuate ligament syndrome is a rare disorder caused by compression of the celiac artery by the diaphragmatic crura, resulting in chronic abdominal pain, often post-prandial in nature. Treatment of this disorder requires skeletonization of the celiac artery. In this study, we used intravenous indocyanine green (ICG) to localize the celiac axis intraoperatively to optimize laparoscopic management in a continuous series of three patients. We then assessed for evidence of clinical improvement. Two of three patients had immediate improvement in chronic abdominal pain (66.7%), and a third patient had an improvement after one week, with sustained improvement at one month follow up (100%) without postoperative complications. In our limited experience, ICG localization of the celiac artery for laparoscopic treatment of the median arcuate syndrome is safe and effective. This novel approach allowed confirmation of complex anatomy and the results in this small series were reassuring.