In this video, we present a case of patient who underwent indocyanine green fluorescence (ICG)-guided laparoscopic cholecystectomy with right trabecular accessory gallbladder removal with intraoperative cholangiography at our institution. The patient was a 57 year-old female with no significant past medical history who presented with possible duplicated gallbladder. Two months prior, she developed 2 episodes of severe biliary colic. She was afebrile and not jaundiced. Her workups with ERCP and MRCP demonstrated an oblong gallbladder with a stone-containing accessory gallbladder consistent with trabecular accessory gallbladder. Accessory gallbladder is a rare congenital anomaly. However, this anatomical variant can be associated with inadequate bile drainage and potentially lead to the development of cholelithiasis. The removal of the accessory gallbladder with cholecystectomy was indicated in this patient due to cholelithiasis and biliary colic. In this case, we demonstrated the surgical technique of laparoscopic cholecystectomy with right trabecular accessory gallbladder removal with intraoperative cholangiography with the utilization of an ICG fluorescence imaging system to visualize and confirm the anatomical variation as well as other structures within the intra- and extra-hepatic biliary system. We found that incorporating the ICG fluorescence imaging system in this particular case facilitated identification of the anatomical variant of the biliary system and helped prevent bile duct injury. The patient successfully underwent the operation with no intraoperative complications. The postoperative course was uneventful and the patient was discharged on postoperative day 1.